https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26867 Wed 11 Apr 2018 17:04:35 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14798 Wed 11 Apr 2018 10:49:42 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33857 Setaria viridis and its close domesticated relative Setaria italica have been chosen as suitable monocotyledonous models for plants possessing the C₄ pathway of photosynthesis including sorghum, maize, sugarcane, switchgrass and Miscanthus×giganteus. Accurate partial least squares regression (PLSR) models to predict S. italica stem composition have been generated, based upon Fourier transform mid-infrared (FTIR) spectra and calibrated with wet chemistry determinations of ground S. italica stem material measured using a modified version of the US National Renewable Energy Laboratory (NREL) acid hydrolysis protocol. The models facilitated a high-throughput screening analysis for glucan, xylan, Klason lignin and acid soluble lignin (ASL) in a collection of 183 natural S. italica variants and clustered them into classes, some possessing unique chemotypes. The predictive models provide a highly efficient screening tool for large scale breeding programs aimed at identifying lines or mutants possessing unique cell wall chemotypes. Genes encoding key catalytic enzymes of the lignin biosynthesis pathway exhibit a high level of conservation with matching expression profiles, measured by RT-qPCR, among accessions of S. italica, which closely mirror profiles observed in the different developmental regions of an elongating internode of S. viridis by RNASeq.]]> Thu 21 Oct 2021 12:52:27 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:10715 Sat 24 Mar 2018 08:08:27 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22245 Solanum lycopersicum L.) lines subjected to normal (control) and heat stress temperatures. At the control temperature of 25/20 °C (day/night) the HT line exhibited higher cell wall invertase (CWIN) activity in flowers and young fruits and partitioned more sucrose to fruits but less to vegetative tissues as compared to the HS line, independent of leaf photosynthetic capacity. Upon 2-, 4-, or 24-h exposure to day or night temperatures of 5 °C or more above 25/20 °C, cell wall (CWIN) and vacuolar invertases (VIN), but not sucrose synthase (SuSy), activities in young fruit of the HT line were significantly higher than those of the HS line. The HT line had a higher level of transcript of a CWIN gene, Lin7, in 5-day fruit than the HS line under control and heat stress temperatures. Interestingly, heat induced transcription of an invertase inhibitor gene, INVINH1, but reduced its protein abundance. Transcript levels of LePLDa1, encoding phospholipase D, which degrades cell membranes, was less in the HT line than in the HS line after exposure to heat stress. The data indicate that high invertase activity of, and increased sucrose import into, young tomato fruit could contribute to their heat tolerance through increasing sink strength and sugar signalling activities, possibly regulating a programmed cell death pathway.]]> Sat 24 Mar 2018 07:17:34 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42812 Mon 05 Sep 2022 10:04:27 AEST ]]>